Identification of introgressed line of BC1F1 from malaysian rice variety / Nur Aqilah Alias

Alias, Nur Aqilah (2015) Identification of introgressed line of BC1F1 from malaysian rice variety / Nur Aqilah Alias. Student Project. Faculty of Applied Sciences, Kuala Pilah, Negeri Sembilan. (Submitted)

Abstract

Rice blast disease is mostly caused by the fungus, Magnaporthe oryzae. Markerassisted backcrossing (MAB) is one of the reasonable approaches to develop a new resistant variety to confront with this challenge. The objective of the study is to identify the introgressed lines from BC1F1 population. A total of 80 seeds from BC1F1 families were obtained from Agrotechnology and Bioprocess Division, Malaysian Nuclear Agency, Bangi, Selangor, Malaysia. Based on the result there are 82.5% of seeds were successfully germinated meanwhile 17.5% seeds were unsuccessfully germinated. DNA was extracted by following the modified CTAB method. PCR analysis showed a pattern of band generated by using RM413 and RM206 primers. Marker RM 413 was tightly linked with pi-21 demonstrated 4 heterozygous (score as “H”) out of 18 backcrossed plants. RM 413 marker can be further used as a foreground marker selection in future generation in development of rice blast resistant variety in Malaysia.

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Metadata

Item Type: Monograph (Student Project)
Creators:
CreatorsID Num.
Alias, Nur AqilahUNSPECIFIED
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > Immunology > Fungal antigens
Q Science > QR Microbiology > Bacteria
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences (N)
Item ID: 23907
Uncontrolled Keywords: Identification of introgressed, line of BC1F1, from malaysian rice variety, UiTM Negeri Sembilan
URI: http://ir.uitm.edu.my/id/eprint/23907

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