Identification of introgressed line of BC1F1 from malaysian rice variety / Nur Aqilah Alias

Alias, Nur Aqilah (2015) Identification of introgressed line of BC1F1 from malaysian rice variety / Nur Aqilah Alias. [Student Project] (Submitted)

Abstract

Rice blast disease is mostly caused by the fungus, Magnaporthe oryzae. Markerassisted backcrossing (MAB) is one of the reasonable approaches to develop a new resistant variety to confront with this challenge. The objective of the study is to identify the introgressed lines from BC1F1 population. A total of 80 seeds from BC1F1 families were obtained from Agrotechnology and Bioprocess Division, Malaysian Nuclear Agency, Bangi, Selangor, Malaysia. Based on the result there are 82.5% of seeds were successfully germinated meanwhile 17.5% seeds were unsuccessfully germinated. DNA was extracted by following the modified CTAB method. PCR analysis showed a pattern of band generated by using RM413 and RM206 primers. Marker RM 413 was tightly linked with pi-21 demonstrated 4 heterozygous (score as “H”) out of 18 backcrossed plants. RM 413 marker can be further used as a foreground marker selection in future generation in development of rice blast resistant variety in Malaysia.

Metadata

Item Type: Student Project
Creators:
CreatorsID Num. / Email
Alias, Nur AqilahUNSPECIFIED
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > Immunology > Fungal antigens
Q Science > QR Microbiology > Bacteria
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Item ID: 23907
Uncontrolled Keywords: Identification of introgressed, line of BC1F1, from malaysian rice variety, UiTM Negeri Sembilan
URI: http://ir.uitm.edu.my/id/eprint/23907

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