HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin

Ag Nuddin, Julenah (2016) HPLC method development & structural elucidation of anti-proliferative quassinoids from Quassia borneensis Noot. (Simaroubaceae) / Julenah Ag Nuddin. In: The Doctoral Research Abstracts. IGS Biannual Publication, 10 (10). Institute of Graduate Studies, UiTM, Shah Alam.


Bitter quassinoids have been reported to possess numerous biological activities. They are degraded triterpenoids classified according to their basic skeletons; C18, C19, C20, C22, C25. Currently, more than 200 quassinoids have been isolated from genus Quassia, Brucea, Soulamea, Eurycoma, Picrolemnia and others. A study on Quassia borneensis Noot. (Simaroubaceae) (Qb) for its anti-proliferative active quassinoids using a developed and validated high performance liquid chromatography (HPLC) isolation method is appropriate for data gathering. In addition, a total synthesis exploration of canthin-6-one would provide information regarding its mechanism and influential factors for viability study. The study was accomplished by extracting powdered bark and root Qb by cold maceration and soxhlet in methanol for yield comparison. The methanol extracts were partitioned to acquire n-hexane, chloroform and aqueous fractions. They were tested for their anti-proliferative activity based on MTS assay using HL-60 cell line. Later, chloroform and aqueous fractions were subjected to isolation and purification with HPLC. Then, pure isolates were structurally elucidated with liquid chromatographymass spectroscopy (LC-MS) and nuclear magnetic resonance (NMR). In addition, the mass profile of active fractions was measured using liquid chromatography-mass spectrometer triple quadrupole time of flight (LC-MSQTOF). Lastly, pure quassinoid compounds were subjected to MTT assay…


Item Type: Book Section
CreatorsEmail / ID. Num
Ag Nuddin, JulenahUNSPECIFIED
Subjects: L Education > LB Theory and practice of education > Higher Education > Dissertations, Academic. Preparation of theses > Malaysia
Divisions: Universiti Teknologi MARA, Shah Alam > Institut Pengajian Siswazah (IPSis) : Institute of Graduate Studies (IGS)
Series Name: IGS Biannual Publication
Volume: 10
Number: 10
Item ID: 19917
Uncontrolled Keywords: Abstract; Abstract of thesis; Newsletter; Research information; Doctoral graduates; IPSis; IGS; UiTM;
URI: http://ir.uitm.edu.my/id/eprint/19917



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