Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian

Sufian, Adila Sahida (2013) Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian. Masters thesis, Universiti Teknologi MARA.

Abstract

In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), ethyl acetate (MCEA) and aqueous (MCAQ)] were subjected to antimicrobial and cytotoxic activities using micro-broth dilution and MTT assays, respectively. MCEA, which appeared to be the most active extract against MSS A (S. aureus 25923) (MIC - 125 μg/mL) and HL60 cell line (human acute promyelocytic leukemia) (IC50 = 17.26 μg/mL), was further fractionated using vacuum liquid chromatography (VLC) to afford seven fractions (F1-F7).

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Metadata

Item Type: Thesis (Masters)
Creators:
CreatorsID Num.
Sufian, Adila SahidaUNSPECIFIED
Divisions: Faculty of Pharmacy
Item ID: 18282
Uncontrolled Keywords: Antimicrobial; Cytotoxic compounds; Muntingia cala
URI: http://ir.uitm.edu.my/id/eprint/18282

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