Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian

Sufian, Adila Sahida (2013) Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian. Masters thesis, Universiti Teknologi MARA.

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Abstract

In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), ethyl acetate (MCEA) and aqueous (MCAQ)] were subjected to antimicrobial and cytotoxic activities using micro-broth dilution and MTT assays, respectively. MCEA, which appeared to be the most active extract against MSS A (S. aureus 25923) (MIC - 125 μg/mL) and HL60 cell line (human acute promyelocytic leukemia) (IC50 = 17.26 μg/mL), was further fractionated using vacuum liquid chromatography (VLC) to afford seven fractions (F1-F7).

Item Type: Thesis (Masters)
Creators:
CreatorsEmail
Sufian, Adila SahidaUNSPECIFIED
Divisions: Faculty of Pharmacy
Item ID: 18282
Uncontrolled Keywords: Antimicrobial; Cytotoxic compounds; Muntingia cala
Last Modified: 07 Feb 2019 00:16
Depositing User: Staf Pendigitalan 5
URI: http://ir.uitm.edu.my/id/eprint/18282

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