Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin

Tahirudin, Muhamad Saifulnizam (2016) Respond of seed culture of Ricinus communis toward different hormone treatment in vitro / Muhamad Saifulnizam Tahirudin. [Student Project] (Unpublished)

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Abstract

This research work aimed to highlight on the effect of various plant growth regulators namely 1-Naphthaleneacetic Acid (NAA) and 6-Benzylaminopurine (BAP) on inducing response toward Ricinus communis in vitro tissue culture. The response observe may be shoot formation, root formation or callus formation that were initiated from seed explant of wild Ricinus communis plant located around Parit Tinggi, Kuala Pilah. Different combinations of hormone treatment were added into MS medium containing sucrose, macronutrient and micronutrient.
Combination of auxin, NAA and cytokinin, BAP gave different response toward the seed explant. After two weeks of inoculation in sterile environment, the response was observed from the seed explant. About 25% of the incubated seed germinated in the MS basal medium. As a result, 0.5, 1.5 and 2.0 mg/1 of NAA combined with 2.5mg/l BAP gave response where callus formation can be observed in this hormone treatment. Shoot formation was observed at 1.5 mg/I NAA and 2.5 BAP and at 1.0, 1.5, 2.0 and 2.5 mg/I NAA combined with 2.0 mg/1 BAP, there was an enlargement of seed explant where the seed enlarged from average of 0.5 cm to 2 cm. In conclusion, different combination of auxin and cytokinin give rise to different response toward the seed explant of Ricinus communis.

Metadata

Item Type: Student Project
Creators:
Creators
Email
Tahirudin, Muhamad Saifulnizam
UNSPECIFIED
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Abu Shah, Nor' Aishah
UNSPECIFIED
Subjects: Q Science > Q Science (General) > Study and teaching > Laboratories. General works
Q Science > QH Natural history - Biology > Life > Growth
Q Science > QK Botany > Plant physiology > Physical plant physiology > Growth. Development. Including pattern formation
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Biology
Item ID: 34502
Uncontrolled Keywords: seed culture, Ricinus communis, hormone treatment in vitro
URI: https://ir.uitm.edu.my/id/eprint/34502

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