Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah

Abdul Hamid Hasani, Narimah and Abdul Kadir, Khalid and Wan Ngah, Wan Zurinah (2011) Palm oil γ-tocotrienol and α-tocopherol act as potent inducers in the immune response of mouse splenocytes in vitro / Narimah Abdul Hamid Hasani, Khalid bin Abdul Kadir and Wan Zurinah Wan Ngah. Scientific Research Journal, 8 (1). pp. 17-28. ISSN 1675-7009

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Official URL: https://srj.uitm.edu.my/

Abstract

Vitamin E may have anti carcinogenesis effect in human and animal models
via the mechanism of cell cycle arrest and enhancement of immune system.
The cell-mediated immune inducing properties of palm oil vitamin E, for
example, γ-tocotrienol and α-tocopherol, were investigated by measuring
the mitogenesis response of splenocytes, extracted from normal male Mus
musculus to splenic T-lymphocytes mitogens, phytohemagglutinin (PHA;
0.25 μg/mL) and concanavalin A (Con A; 1.0 μg/mL); and B-lymphocytes
mitogen i.e., lipopolysaccharide (LPS; 1.0 μg/mL). Both γ-tocotrienol
and α-tocopherol enhanced the cell proliferation of mitogen untreated
splenocytes as determined by 5-Bromo-2'-deoxy-uridine (BrdU) detection
method. Both compounds also enhanced the T-lymphocytes response to PHA
and Con A, as well as B-lymphocytes responses to LPS at all concentration
used (0-300 μM). γ-Tocotrienol was observed to affect cell proliferation
more than α-tocopherol. The uptake of γ-tocotrienol and α-tocopherol into
the splenocytes was determined by high performance liquid chromatography
(HPLC). γ-Tocotrienol was absorbed into the cells at markedly higher
levels than α-tocopherol with the ratio of 4.8 : 1 (p<0.01, n=4) at 300
μM of treatment. This may be the reason of the higher proliferation affect
of γ-tocotrienol as compared to α-tocopherol. In conclusion, we are
of the opinion that palm oil γ-tocotrienol and α-tocopherol are able to synergistically influence splenocytes proliferation thus enhancing the cellmediated
immune system.

Metadata

Item Type: Article
Creators:
Creators
Email
Abdul Hamid Hasani, Narimah
drnarimah@salam.uitm.edu.my
Abdul Kadir, Khalid
UNSPECIFIED
Wan Ngah, Wan Zurinah
UNSPECIFIED
Subjects: Q Science > QP Physiology > Animal biochemistry > Tocotrienol. Tocopherol. Vitamin E
Q Science > QR Microbiology > Immunology > Immune response
Divisions: Universiti Teknologi MARA, Shah Alam > Research Management Centre (RMC)
Journal or Publication Title: Scientific Research Journal
UiTM Journal Collections: UiTM Journal > Scientific Research Journal (SRJ)
ISSN: 1675-7009
Volume: 8
Number: 1
Page Range: pp. 17-28
Official URL: https://srj.uitm.edu.my/
Item ID: 12942
Uncontrolled Keywords: Gamma-tocotrienol, α-tocopherol, splenocytes, immune response
URI: https://ir.uitm.edu.my/id/eprint/12942

ID Number

12942

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