Analysis of the total phenolic content from different species of grapes by using pulp and skin in two different solvents; water and methanol / Muhd Farizul Md Ismail

Md Ismail, Muhd Farizul (2016) Analysis of the total phenolic content from different species of grapes by using pulp and skin in two different solvents; water and methanol / Muhd Farizul Md Ismail. [Student Project] (Unpublished)

Abstract

Nowadays, study in total phenolic content from natural products has been actively carried out. As a raw material, different species of grapes was chosen. This study was designed to analysis of the total phenolic content from different species of grapes by using pulp and skin in two different solvents; water and methanol. The total phenolic content was determined by using the Folin Ciocalteu assay. Gallic acid was selected as the standard in order to determine the calibration curve of TPC. The grapes were extracted with methanol according to ratio 4:6 while the others grapes were extracted with distilled water and both flasks were incubated in 30 minutes for further analysis. The experiment was conducted in UiTM Negeri Sembilan laboratory by using UV-visible spectrophotometer. From this study, it indicated that black grapes species contain the highest value of TPC which is 8.963 mg GAE/L than red and green grape varieties in all parts of grapes studied. Methods validation is also evaluated under the factor of linearity and calibration, limit of detection, limit of quantification, laboratory fortified blank, laboratory reagent blank and precision. For the gallic acid calibration curve, the linear coefficient obtained is 0.9999 which is absorbance is directly proportional to the concentration. The value of LOD, LOQ, LRB and LFB is 0.069 ppm, 0.076 ppm, 3.1123 mg GAE/L ± 0.6655 and 19.9790 mg GAE/L ± 0.7185. Besides that, the percentage of precision achieved was 119.1% which indicated that the results was in the favorable range.

Metadata

Item Type: Student Project
Creators:
Creators
Email / ID Num.
Md Ismail, Muhd Farizul
UNSPECIFIED
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Ahmad, Nor Monica
UNSPECIFIED
Subjects: Q Science > Q Science (General) > Study and teaching > Laboratories. General works
Q Science > QD Chemistry > Physical and theoretical chemistry > Chemical elements
Q Science > QD Chemistry > Extraction (Chemistry)
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Chemistry
Keywords: Analysis, total phenolic content, grapes, pulp, skin, solvents water, methanol
Date: 2016
URI: https://ir.uitm.edu.my/id/eprint/45292
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