Antimicrobial activity of chitosan prepared from Sepia cuttlebone against pathogen causing skin infection / Noor Atikah Jailani

Cuttlebone is the internal structure from the species in the family Sepidae. This research was performed to determine the antimicrobial activity of chitosan prepared from Sepia cuttlebone against Staphylococcus aureus, Escherichia coli and Candida albicans by using disk diffusion method and minimum inhibitory concentration (MIC). Result from Fourier Transform Spectroscopy Infrared (FTIR) proved the presence of chitosan in cuttlebone extract that acts as bioactive compound in this antimicrobial research. In extraction of chitosan from Sepia cuttlebone, it involve three step which is demineralization, deproteinization and deacetylation. For this research, two concentration of chitosan extract have been used in disk diffusion method, which is 100mg/ml and 50mg/ml to determine the inhibition zone against S.aureus, E.coli and C.albican. For MIC, the extract is investigated at 20,40,60,80 and 100 mg/ml concentration. The result of this research showed the chitosan extract have high antifungal activity compared to antibacterial activity.