Identification of tentative antioxidant constituents from stem and leaves of leea indica species using spectroscopic analysis / Fatin Azieyati Baharom …[et al.]

Baharom, Fatin Azieyati and Mohd Azam, Naila Nabihah and Harun, Aiza and Daud, Shaari and Mat So’ad, Siti Zaiton and Mohd Hassan, Norazian (2020) Identification of tentative antioxidant constituents from stem and leaves of leea indica species using spectroscopic analysis / Fatin Azieyati Baharom …[et al.]. Gading Journal for Science and Technology, 3 (1). pp. 129-136. ISSN 2637-0018

Abstract

The tentative antioxidative constituents from stem and leaves extracts of Leea indica which
traditionally known as Mali-mali had been investigated. Three different polarities of crude
extracts of each part was prepared using consecutive soaking with petroleum ether,
dichloromethane and methanol. Screening of antioxidants of each extract on TLC was
performed with the use of 2,2 diphenyl-1-picrylhydrazyl (DPPH) as spraying reagent and
have been previously reported. The isolation of antioxidative compounds was performed
through preparative thin layer chromatography(pTLC) with the use of certain binary solvents
system. The structure determination was carried out using Fourier Transform Infra-Red
(FTIR), Gas Chromatography Mass Spectrometer (GCMS) and Proton Nuclear Magnetic
Resonance (NMR) along with comparison with literature. FTIR analysis depicted some
important functional groups such as OH, C-H, C=C and C-O stretching in both parts. The
C=O and C-N stretching only appeared in leaves extract of L. indica. According to proton
NMR spectrum of leaves part, the chemical shifts belonged to aliphatic protons, vinylic
protons, hydroxyl proton and aromatic protons. The proton NMR spectrum of stem part
revealed the existence of proton aliphatic, vinylic proton and hydroxyl proton. Based on
GCMS analysis with the assistance of Wiley 275.L database matching individually as well as
with proton NMR data, the antioxidative compounds isolated from stem part were proposed
as 1-(hydroxymethyl)-1,2- ethanediyl ester (LI-1). 9-Oxononanoic acid methyl ester (LI-2),
9,12-Octadecadienoic acid (LI-3), 3’,8,8’-Trimethoxy-3-piperidyl-2,2’-binaphthalene1,1’,4,4’-tetrone (LI-4).

Metadata

Item Type: Article
Creators:
Creators
Email / ID Num.
Baharom, Fatin Azieyati
aizaharun@uitm.edu.my
Mohd Azam, Naila Nabihah
UNSPECIFIED
Harun, Aiza
aizaharun@uitm.edu.my
Daud, Shaari
shaari111@uitm.edu.my
Mat So’ad, Siti Zaiton
dszaiton@iium.edu.my
Mohd Hassan, Norazian
norazianmh@iium.edu.my
Subjects: Q Science > QD Chemistry > Extraction (Chemistry)
Q Science > QD Chemistry > Analytical chemistry
Q Science > QK Botany > Plant physiology > Botanical chemistry. Phytochemicals
Divisions: Universiti Teknologi MARA, Pahang > Jengka Campus
Journal or Publication Title: Gading Journal for Science and Technology
UiTM Journal Collections: UiTM Journal > Gading Journal of Science and Technology (GADINGS&T)
ISSN: 2637-0018
Volume: 3
Number: 1
Page Range: pp. 129-136
Keywords: Leea indica, Antioxidant, Secondary Metabolites, proton NMR
Date: 31 March 2020
URI: https://ir.uitm.edu.my/id/eprint/31120
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