Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama

Abd Rahim, Siti Noraida and Sulaiman, Alawi and Edama, Nurul Aini (2014) Kinetics of tapioca slurry saccharification process using immobilized multi-enzyme system enhanced with Sg. Sayong clay / Siti Noraida Abd Rahim, Alawi Sulaiman and Nurul Aini Edama. Journal of Mechanical Engineering, 11 (2). pp. 67-77. ISSN 1823-5514

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Abstract

In this paper, immobilization of multi-enzyme using calcium alginate-clay beads for tapioca slurry saccharification process was carried out. The kinetics parameter and immobilization yield for alpha-amylase, glucoamylase, cellulose and multi-enzyme system were compared. The Michaelis constant, Km for free alpha-amylase, glucoamylase, cellulase and multi-enzyme were 2.0831,1.8326, 7.8592 and 3.5367 mg/mL, respectively. As for the immobilized system, the Km values were 3.1604, 2.1708, 9.2791 and 4.91176 mg/mL, respectively. The Km value for the immobilized multi-enzyme system was 1.39 times higher than free multi-enzyme system. This suggests that the immobilized multi-enzyme is a potential system for tapioca saccharification process due to affinity of enzyme to the substrate. For the immobilization yield, the yield for alpha-amylase, glucoamylase, cellulase and multi-enzyme were 51.19, 50.21, 40.68 and 53.71 %, respectively. The result showed the immobilized multi-enzyme had a higher yield compared to the single enzyme which could be explained due to the synergistic action of alpha-amylase, glucoamylase and cellulose enzymes. Therefore, it can be concluded that the use of immobilized multi-enzyme system had a very good potential for starch bioconversion into glucose.

Item Type: Article
Uncontrolled Keywords: Immobilization, calcium alginate-clay beads, multi-enzyme, tapioca slurry, kinetic parameter, immobilization yield
Subjects: T Technology > TA Engineering. Civil engineering > Engineering mathematics. Engineering analysis
Divisions: Faculty of Mechanical Engineering
Depositing User: Staf Pendigitalan 5
Date Deposited: 24 Aug 2017 01:50
Last Modified: 24 Aug 2017 01:50
URI: http://ir.uitm.edu.my/id/eprint/17650

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